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#QPCR
Michael Mina
michaelmina_lab
THREAD: Maybe you’ve heard about Ct values in the qPCR test and viral loads. So what’s a Ct value, why is higher Ct a lower viral load, what’s it have
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Raul Gonzalez
PoopScience
I'm convinced ddPCR is more sensitive wrt environmental samples than qPCR. Many assays that we use on the digital platform were designed qPCR but could ddPCR be more sensitive (in
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Michael Mina
michaelmina_lab
The massive confusion created by the media and the announcement of SalivaDirect for #COVID19 testing needs to be cleared up. Yesterday I wrote a long tweet thread about it. The
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Kevin McKernan 🙂
Kevin_McKernan
This is the Final nail in the coffin of the current qPCR testing strategy.We have to get smarter about what we are targeting and stop harassing asymptomatic people.Now, let’s imagine
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Jonathan Cedernaes
JCedernaes
A rather new #SARSCoV2 variant B.1.525 has been identified in some countries, e.g. England & the U.S.Id dates in photo below from https://cov-lineages.org/global_report_B.1.525.html.There is however no evidence so far that
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Kevin McKernan 🙂
Kevin_McKernan
Freedom Requires Live-Dead analysis: Subgenomic RNAYou need to pay attention to Wolfel et al and Kim et al.A very elegant method for Live-Dead analysis.But before we dive into that Mol-Bio
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Michael Mina
michaelmina_lab
Terrific news that SalivaDirect gained @US_FDA approval today! A major step forward for lab based #COVID19 diagnostics. I'll explain why this is good for DIAGNOSTICS but also why this is
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Kevin McKernan 🙂
Kevin_McKernan
Someone has reading comprehension problems.Where does our document say this?Starting off with a straw man is a sign of deception.We are quite clear, that they have no clue what their
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Kevin McKernan 🙂
Kevin_McKernan
Cliff Notes of 4 papers that Dissolve the Corman-Drosten RT-qPCR test. https://twitter.com/Kevin_McKernan/status/1335357508618252289?s=20 We'll start with Muenchhoff et al. published in Eurosurveillance which concludes with Charite'
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Kevin McKernan 🙂
Kevin_McKernan
Let's walk through the much more complicated problem of identifying pathogens on Cannabis flower. These are inhaled products in an industry that just experienced EVALI.This will provide a few examples
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Kevin McKernan 🙂
Kevin_McKernan
Evidence of rigged Peer Review @Eurosurveillanc @PlanetWaves @Bobby_Network @RetractionWatch @goddeketal Admits to being the reviewer of the Corman-Drosten Retraction request.This is 60+ pages discussing Primer Dimers and other primer desig
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Andrew💙Croxford
andrew_croxford
NEW THREAD: But one with a potentially small target audience, namely front-line #NHS staff of all stripes who have had SARS-CoV-2 infection at some point during the pandemic, and are
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Kevin McKernan 🙂
Kevin_McKernan
1\But but... you ran it to 40-45 cycles? This is an argument that needs dissection. The protocols you see in EUAs are speaking to the Camera time not the Call
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Kevin McKernan 🙂
Kevin_McKernan
I've been asked this many times. So tweet thread it is.Do the Drosten primers hit the human genome?Why do some people claim they hit 100%?TL/DR: Here is the output from
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Amy Proal, PhD
microbeminded2
This is an incredibly important preprint to inform #LongCovid. Among many analyses, the team recruited 4 patients w/ prolonged + recurrent olfactory function loss after #COVID-19 (time from first COVID-19
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Phil Kerpen
kerpen
NATURE: "We tested 690 respiratory samples from the 129 patients for the presence of infectious virus using cell culture and determined the viral RNA load with RT-qPCR (Fig. 1). Infectious
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