This was a long tough road for many reasons (long revision experiments with LLSM, limited computer resources then, a change in job, a lab move, and the COVID-19 following that), but many good things out of this work. https://www.nature.com/articles/s42003-021-01740-y#Sec22
I am glad I have a team that I only saw getting tougher through some genuinely frustrating times to see this through - the then honors student @HarrisonMYork took the project up in its infancy, with contributions from Ullhas(not on twitter), @abhishekworld, Aditi and Hetvi.
Big thanks to @SingMolSci, @UNSWMedicine for the initial springboard, @NerdyCammy of @MIPS_Australia & @MMI_Clayton for access to equipment and @Monash_FMNHS & @MonashBDI for the continued support!
One of the important things that came out of this work for us, was that we started thinking about endosomal dynamics, receptor management and signalling dynamics more analytically. https://portlandpress.com/biochemsoctrans/article/48/5/2051/226395/To-be-more-precise-the-role-of-intracellularsignalingThis is where our lab @cellphy (cellular physiology) is headed!
At @MonashBDI and @EMBLAustralia, our lab, @cellphy aims to understand how do cells measure, what is their precision & sensitivity, what roles do endosomes play, and can they determine/affect tissue level temporal processes?
But @HarrisonMYork you can relax, for a bit ;)