Read on Twitter
prePAPER ALERT
@ScherrMatthias @MPI_Biochem ft. @LabRemus @syafiqabdwahab @sloan_kettering 
Born to Slide: Mobile Origin Licensing Factors Confer Resistance to Transcription Conflicts
http://cutt.ly/lkoxCnf #singlemolecule
observations starring MCMs & RNApol - a 
Not only do 
MCMs slide on their own, 
RNA polymerase can push them around on DNA!
Interested in what happens with nucleosomes in that scenario?
MCMs slide on their own, RNA polymerase can push them around on DNA!Interested in what happens with nucleosomes in that scenario?
So were we! Here the nucleosome is shown in pink 
What we see is that not only can RNA polymerase push MCMs, but upon encountering a nucleosome they keep on sliding all together - the mobile super-complex.
But where is Origin Recognition Complex (ORC) in the story you ask?
What we see is that not only can RNA polymerase push MCMs, but upon encountering a nucleosome they keep on sliding all together - the mobile super-complex.
But where is Origin Recognition Complex (ORC) in the story you ask?
Here shown in green, ORC is being pushed by RNA polymerase.
At least, that is what happens most of the time...
At least, that is what happens most of the time...
However, from time to time, RNA polymerase can simply bypass ORC, which stays bound to the origin 
#plasticity
But what happens when ORC is in the company of the helicase?
#plasticityBut what happens when ORC is in the company of the helicase?
The helicase loading intermediate ORC-Cdc6-Cdt1-Mcm2-7 (OCCM) doesn't present an obstacle for RNA polymerase. Seems like RNA polymerase is the 
that just keeps going!
Does it ever stop?
that just keeps going!Does it ever stop?
There is one place where RNA polymerase will stop - at the terminator site 
(here in the middle of the frame). Upon reaching the terminator site, RNA polymerase disengages from DNA, while ORC-Cdt1-MCM complex remains stable 
(here in the middle of the frame). Upon reaching the terminator site, RNA polymerase disengages from DNA, while ORC-Cdt1-MCM complex remains stable
All together, we show that licensing pathway is actually very dynamic - a feature which might provide much needed resistance of replication and transcription machineries to diverse obstacles encountered on chromosomes 
This is our first Sneak Peek - a preview of paper under review in Cell Press research journal pipeline @SSRN @cellpressnews
