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Excited to share that the last piece of my PhD work in the @MauckLab is out now in @Biomaterials_ , where we further explore the role of nuclear envelope wrinkling in mechanotransduction! A few of our main findings from the paper below (1/n): https://www.sciencedirect.com/science/article/abs/pii/S0142961221000132?via%3Dihub
(2/n): Point 1: Nuclear envelope morphology is rapidly remodeled by changes in cellular contractility
(3/n): Nuclear envelope laxity stored in wrinkles must be removed prior to robust mechano-responses, and thereby functions as a mechanical buffer. This aligns nicely with observations/modeling from @andrewdstephens / @Irate_Physicist / @JohnFMarko in 2017.
(4/n): These observations further support the idea of of the nuclear lamina as a mechanical shock absorber that @krisnoeldahl described in 2004. (thanks @brianneconnizzo for the extra image!)
(5/n): Removal of nuclear envelope laxity by the actomyosin cytoskeleton happens in a markedly different fashion in 2D vs. 3D environments.
(6/n): This increased nuclear wrinkling in pro-contractile 3D environments is largely driven by impingement from actin filaments in many directions in these 3D environments. These impingements could range from slight surface impingements to increasingly deeper impingements.
(7/n): A quick summary image. Our observations support other cool work out recently on the wrinkled nuclear morphology in different contexts from (Michele Nava/ @KateMiro1 / @WickstromLab), ( @valeria_ventur / @Ruprecht_Lab /Weiser Lab), ( @Alexis_Lomakin/ @PielMatthieu) and others!
(8/8): Thank you so so much to all of our collaborators @BurdickLab @DymentLabPenn @TrisDris @TTsinman @EricDai_BioE @HeoLab, & esp @ClaudiaLoebel who was instrumental in finishing this work! I couldn't have worked on this with a more fun and supportive group of people :).
