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Great to see our paper on micropatterning with fibrinogen anchors online in @JCellBiol!
http://bit.ly/3q6vipv
A thread... 1/9
http://bit.ly/3q6vipv
A thread... 1/9
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In this paper we improve the printing of proteins ('micropatterning') by developing a universal (fibrinogen) micropatterning anchor. This allows several cool new applications:
In this paper we improve the printing of proteins ('micropatterning') by developing a universal (fibrinogen) micropatterning anchor. This allows several cool new applications:
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Micropatterning experiments are often hindered by an inability to 'print' certain proteins well.
Micropatterning experiments are often hindered by an inability to 'print' certain proteins well.
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By fusing fibrinogen, a protein that 'prints' well, to common binders (e.g. nanobodies, NeutrAvidin, biotin), we can then bind any tagged protein to these high density, homogenous micropatterns, improving the micropatterning of certain proteins.
By fusing fibrinogen, a protein that 'prints' well, to common binders (e.g. nanobodies, NeutrAvidin, biotin), we can then bind any tagged protein to these high density, homogenous micropatterns, improving the micropatterning of certain proteins.
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Binding proteins to micropatterned fibrinogen anchors after the micropatterning process also allows us to protect fragile proteins from the potentially damaging micropatterning process. For example, we can now micropattern microtubule motors in an active state!
Binding proteins to micropatterned fibrinogen anchors after the micropatterning process also allows us to protect fragile proteins from the potentially damaging micropatterning process. For example, we can now micropattern microtubule motors in an active state!
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Because of these improvements, that allow robust, high density printing of any protein, we can take micropatterning to the next level and go subcellular!
Because of these improvements, that allow robust, high density printing of any protein, we can take micropatterning to the next level and go subcellular!
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Here, we constrain the shape of a cell with an outer ring of cell adhesion molecules, and then relocalise receptors to the central disc by fibrinogen-anchored printing of their cognate ligand.
Here, we constrain the shape of a cell with an outer ring of cell adhesion molecules, and then relocalise receptors to the central disc by fibrinogen-anchored printing of their cognate ligand.
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This subcellular micropatterning will allow us, for example, to untangle polarised receptor signalling and polarised cell adhesion forces.
This subcellular micropatterning will allow us, for example, to untangle polarised receptor signalling and polarised cell adhesion forces.
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We hope you enjoy the paper! This was a really great collaboration with the Chin and Blacklow labs! @_JosephWatson @aichsamya @BOllerSalvia
We hope you enjoy the paper! This was a really great collaboration with the Chin and Blacklow labs! @_JosephWatson @aichsamya @BOllerSalvia
