Some basics on T-PLL; I was reviewing e-lab modules from Institute for Quality Management in Healthcare (IQMH), and found their collection of educational material useful. /1
  #hemepath #flowcytometry #tcell #TPLL

image from Blebs in T-PLL cells ( http://hematology.org ) 1/

Morphology:
Small to medium-sized lymphoid cells with mature chromatin, non-granular basophilic (blue) cytoplasm, round or oval nuclei with sometimes a visible nucleolus and cytoplasmic blebs 
I can’t help seeing a face 2/

T-cell prolymphocytic leukemia (T-PLL), a rare, aggressive mature T-cell neoplasm of adults >30 years with poor prognosis and short survival. 

Clinical feature: 
Marked leukocytosis, anemia, thrombocytopenia, 
Hepatosplenomegaly, rash, effusion 3/

Morphological variant - can have cerebriform nuclei 4/

Here is how Sézary cells typically look like with prominently folded "cerebriform" nuclei shown in Image Bank Sézary Cells - ( http://hematology.org ) 5/

Sharing the IQMH link and my notes on essential plots /2

Institute for Quality Management in Healthcare. (2020). E-Learn LAB. https://iqmh.org/Services/Centre-For-Education/eLearn-LAB/Flow-Cytometry 

Plotsshow expression of pan T antigens  
NK antigens: CD16/CD56/CD57 ( not shown) are usually neg 6/

(right )Pro-lymphocytes are also evaluated for markers of immaturity, including CD1a, CD34, and TdT, TPLL shows no expression, allowing distinction from immature T-cell neoplasms 7/

Pears to Remember 8/

@IR_AMauntie @TashakoriM @AmlLabib @kitagawa_yukiko @Gagandeepk5MD @RitchaSaxena @RyanneMDMBA @albis49 @syeda_qasim @javidiparsijani @AlanPath @dr_aliraza @xpn007 @DrBMcGinn

Here is an awesome case of T-PLL presented by #SocHemepath #SHQuickbyte
  https://twitter.com/SocforHemepath/status/1320724488162578434?s=20