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Hey #STED, #MINFLUX people,
To continue the public discussion, I list here some of the points I made during my talk.
Summary: MINFLUX is a promising technology but at an early stage of development esp for biological research.
Thread
cc @Stefan_W_Hell @BalzarottiFran
To continue the public discussion, I list here some of the points I made during my talk.
Summary: MINFLUX is a promising technology but at an early stage of development esp for biological research.
Thread
cc @Stefan_W_Hell @BalzarottiFran
Disclaimer 1: I haven't seen or worked with #MINFLUX setup, my arguments mainly on application-side
Disclaimer 2: I asked these questions via email (Sept 2020) and requested raw data and codes but haven't received a reply yet
I hope @rita_strack @naturemethods can help!
Disclaimer 2: I asked these questions via email (Sept 2020) and requested raw data and codes but haven't received a reply yet
I hope @rita_strack @naturemethods can help!
You can learn more about #MINFLUX from this excellent talk by @Stefan_W_Hell
Also, some papers making an effort in this direction
Also, some papers making an effort in this direction
"MINFLUX imaging of nuclear pore complexes of a mammalian cell shows that this true nanometer-scale resolution is obtained in three dimensions and in two color channels"
Let's examine this claim but first what are nuclear pores and their dimensions?
Let's examine this claim but first what are nuclear pores and their dimensions?
I just noticed that the original thread breaks here
Remaining thread with 8 points on #MINFLUX here https://twitter.com/kirtiprakash25/status/1336579243313156101
Remaining thread with 8 points on #MINFLUX here https://twitter.com/kirtiprakash25/status/1336579243313156101
Microscope users, facility managers,
What biological applications of #MINFLUX u can think of to convince the Institute head to invest in one?
@KRogers1001 @manorlaboratory @sossick @AlvaroCrevenna @StefanieReiche6 @koholleran @JenCWaters @YorkBioimaging @EllenbergLab @AICjanelia
What biological applications of #MINFLUX u can think of to convince the Institute head to invest in one?
@KRogers1001 @manorlaboratory @sossick @AlvaroCrevenna @StefanieReiche6 @koholleran @JenCWaters @YorkBioimaging @EllenbergLab @AICjanelia
I start.
I would try to resolve nucleosomes!
Unlike nuclear pores, it is a continuous structure and about 8-10 nm.
So would test the performance of #MINFLUX on biological structures < 10 nm.
Also, any excessive filtering would result in a discontinuous, undersampled structure.
I would try to resolve nucleosomes!
Unlike nuclear pores, it is a continuous structure and about 8-10 nm.
So would test the performance of #MINFLUX on biological structures < 10 nm.
Also, any excessive filtering would result in a discontinuous, undersampled structure.
Recording of my talk on #MINFLUX and super-resolution microscopy @RoyalMicroSoc
MINFLUX starts ~16 min
Loved the discussion session with @alesposito75 @StefanieReiche6 @sossick
Also, surprised to have more views in 1 day than Chris Dunsby in 3 weeks!
MINFLUX starts ~16 min
Loved the discussion session with @alesposito75 @StefanieReiche6 @sossick
Also, surprised to have more views in 1 day than Chris Dunsby in 3 weeks!
I have received >15 messages across different platforms last days briefly saying something like - "I like the points you make abt MINFLUX but I prefer to be on the safe side"
Fine if you want to be the silent spectator but pls don't forget the popcorn, more stuff to come
!
Fine if you want to be the silent spectator but pls don't forget the popcorn, more stuff to come
!
In general, scientific papers are hard to write but this one was the hardest I have written!
Happy to see ~300 downloads, ~500 views in one day across different platforms!
A lot of effort went in, please do read and comment!
https://www.preprints.org/manuscript/202102.0173/v1
https://twitter.com/kirtiprakash25/status/1358679139595800578
Happy to see ~300 downloads, ~500 views in one day across different platforms!
A lot of effort went in, please do read and comment!
https://www.preprints.org/manuscript/202102.0173/v1
https://twitter.com/kirtiprakash25/status/1358679139595800578
Might come as a surprise but along with Ernst Stelzer and Eric Betzig, Stefan Hell is one of my fav writers!
Some memorable quotes from the MINFLUX papers (Science 2017, Nat Meth 2020)
Some memorable quotes from the MINFLUX papers (Science 2017, Nat Meth 2020)
