(1/9) Thrilled to share that the latest paper from @FarinasLab has been released online today in @CellStemCell!! Something we’ve been working on for quite a while, so it’s a rush to see it finally out.

(2/9) Thanks Germán Belenguer, @_anadomingo_, @PereDuart, @ToniJordanPla, @Laublasch, @SacriFerron and @IsabelFarinas for the great team effort and not waver a bit, not even having to go through revision during the past months #confinement.

(3/9) Proud to present a versatile FACS panel to analyse the whole subependymal neurogenic lineage, including neural stem cells at different proliferative states: deeply quiescent (qNSC), shallow quiescent but primed-for-activation (pNSC) and actively dividing (aNSC).

(4/9) Application of this panel does not require the use of reporter transgenic mice and segregates all the populations with a resolution only achieved so far by single-cell approaches. Already applying it to most of our models!

(5/9) Wanting to fill the gap between single cell assays and classic immunofluorescence studies, it is also combinable with #EdU, so typical #Label_retaining experiments can be re-examined at much higher resolution (and just in a few hours!).

(6/9) Faithful believers in the #neurosphere culture, we’ve found that they are less heterogeneous than widespread thought, and contain slow-cycling primed-like NSC (even with a bunch of mitogens knocking on their membranes!).

(7/9) Always interested in the #niche and its role in NSC biology, this time we have gone beyond the #SEZ boundaries to see that systemic inflammation actively regulates NSC #quiescence. No doubt we are at the dawn of #primedNSC era with so much yet to explore.

(8/9) Hope our work helps reconcile contradictory results of inflammation effects on #neurogenesis. We found that TNFα, acting selectively through #TNFR1 and #TNFR2 alerts #primedNSC to a transient non-productive activation that ends in a return to deep #quiescence.

(9/9) Looking back at the whole story, perhaps we should have honoured the great #Tolkien and title the paper “There and back again: A #primedNSC tale”. (Dead sure it sometimes felt like crossing to Mordor!).

Check it out here: https://www.cell.com/cell-stem-cell/fulltext/S1934-5909(20)30510-5?rss=yes