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Are you interested in mouse models of #stroke? And their suitability to study blood-brain-barrier #BBB disruption? Please have a look at our recent work published @FrontPhysiol (+ my first paper as a senior author! 
) a little tweetorial below 
. 1/7 https://www.frontiersin.org/articles/10.3389/fphys.2020.586226/full?&utm_source=Email_to_authors_&utm_medium=Email&utm_content=T1_11.5e1_author&utm_campaign=Email_publication&field=&journalName=Frontiers_in_Physiology&id=586226
) a little tweetorial below . 1/7 https://www.frontiersin.org/articles/10.3389/fphys.2020.586226/full?&utm_source=Email_to_authors_&utm_medium=Email&utm_content=T1_11.5e1_author&utm_campaign=Email_publication&field=&journalName=Frontiers_in_Physiology&id=586226
Different mouse models are used to study stroke pathophysiology + to evaluate treatments. Apart from MCAO, the PT stroke is an ever-increasingly used model with well-characterized motor deficits. However, BBB injury has not been studied beyond the acute stage in this model. 2/7
Mice received a PT stroke in the sensorimotor cortex and were injected with a BBB permeability marker. We determined that most damage and vascular permeability in the PT stroke is present at the site of injury 1dpi. Later, most of the animals still exhibit enhanced leakage. 3/7
Other brain regions, which were not directly affected by the initial stroke, also displayed enhanced although lower leakage in some animals. This has been previously observed in patients and linked to degeneration in projection areas + input systems to the stroke region. 4/7
The observed BBB disruption is associated with substantial anatomical damage to major tight and adherens junction proteins and cellular components of the neurovascular unit, especially pericytes. 5/7
Taken together, the photothrombotic stroke shows many features of BBB disruption that have been previously observed in stroke patients and other rodent stroke models. It therefore represents a suitable model to study BBB pathology. 6/7
I am very grateful to everyone who contributed @uzh_irem @CTackenberg @UZH_en. Especially don't forget to congratulate the talented @RebeccaZ_Weber for her first first-author paper! With many more exciting stories to come 
. Please feel free to give feedback and retweet. 7/7
. Please feel free to give feedback and retweet. 7/7
