Read on Twitter
Check out our new paper @molecology
https://onlinelibrary.wiley.com/doi/abs/10.1111/mec.15609?af=R
on #wolbachia interactions with an #insect-specific virus in #mosquitoes in natural populations
Many thanks to co-authors:
@sebastianlequime, @CelestineAtyame, F. Justy, M.Weill & M.Sicard
https://onlinelibrary.wiley.com/doi/abs/10.1111/mec.15609?af=R
on #wolbachia interactions with an #insect-specific virus in #mosquitoes in natural populations
Many thanks to co-authors:
@sebastianlequime, @CelestineAtyame, F. Justy, M.Weill & M.Sicard
This is also the last paper from my PhD so a little thread including some other related papers to celebrate
Many Insect-Specific Viruses(ISV) found in mosquitoes. Some shown to interfere with arboviruses hence are promising for arbovirus control.
But many unknowns on ISV transmission & interactions with the rest of the mosquito microbiota
https://doi.org/10.3390/v11090873
by @agboli_eric et al
But many unknowns on ISV transmission & interactions with the rest of the mosquito microbiota
https://doi.org/10.3390/v11090873
by @agboli_eric et al
Culex pipiens densovirus (CpDV) is an insect-specific virus & widespread in Culex pipiens complex mosquitoes https://academic.oup.com/ve/article/5/2/vez053/5648224
And vertically transmitted along with Wolbachia in lab colonies of C. pipiens https://sfamjournals.onlinelibrary.wiley.com/doi/abs/10.1111/1462-2920.14511
Here we focused on Culex pipiens densovirus (CpDV) and natural Wolbachia of C. pipiens complex mosquitoes (wPip) and their interactions in natural C.pipiens populations in Northern Tunisia
wPip can be grouped in 5 genetically distinct groups and Atyame et al. 2015 describes a stable coexistence zone where 2 of these groups, wpip-I and wpip-IV coexist. https://onlinelibrary.wiley.com/doi/abs/10.1111/mec.13035
Since all C. pipiens are infected with wPip, we focused on this area to study the effect of different wPip groups on CpDV
In the sites, where both wPip groups and CpDV coexisted (same ecological conditions & same chance to get CpDV infection)
- Larvae with wPipIV were more likely to have CpDV
- & we observed a positive correlation between wPip and CpDV amounts (but no effect of wPip group on this)
- Larvae with wPipIV were more likely to have CpDV
- & we observed a positive correlation between wPip and CpDV amounts (but no effect of wPip group on this)
We then sequenced CpDV from the samples to check for vertical cotransmission with wPip groups: If they are strictly vertically cotransmitted with wPip, we can expect a phylogenetic signal linked to wPip groups in CpDV phylogeny
We saw this signal only in the areas where only one wpip group existed. where both wPip groups coexisted no difference between randomized and real data, suggesting CpDV horizontal transmission in these areas in addition to its previously shown vertical transmission
Virus-Wolbachia interactions are studied mostly for +ssRNA viruses & mostly for arboviruses where Wolbachia interferes with virus infection. In contrast Wolbachia seems to increase densovirus replication (check also this paper from @RhysHParry et al ) https://www.sciencedirect.com/science/article/pii/S0042682218303702?via%3Dihub
Mechanisms behind Wolbachia-virus interference and what causes the differences bt different viruses are still unknown but cool review about possible mechanisms by
@Amelia_Lindsey et al. https://www.mdpi.com/1999-4915/10/4/141
@Amelia_Lindsey et al. https://www.mdpi.com/1999-4915/10/4/141
In summary, Wolbachia can affect the distribution and evolution of insect-specific viruses (and vice versa), especially the ones that are vertically transmitted, by interfering with or facilitating their replication or transmission. And there are still lots to learn!
