"can you link to evidence that the virus was isolated" is becoming fake moon landing level stupid
Study1: 23JAN2020
https://www.biorxiv.org/content/10.1101/2020.01.22.914952v2
🧫bronchoalveolar lavage fluid from RT-PCR-positive patient (ICU-06 in Spike tree=WIV04/2019 virus)
🧫successfully isolated nCoV-2019
124 BetaCoV/Wuhan/WIV04/2019 in Vero and Huh7 cells
🦠cytopathic effects (CPE) seen
Study1: 23JAN2020 https://www.biorxiv.org/content/10.1101/2020.01.22.914952v2bronchoalveolar lavage fluid from RT-PCR-positive patient (ICU-06 in Spike tree=WIV04/2019 virus)successfully isolated nCoV-2019124 BetaCoV/Wuhan/WIV04/2019 in Vero and Huh7 cellscytopathic effects (CPE) seen
Study1: 23JAN2020 https://www.biorxiv.org/content/10.1101/2020.01.22.914952v2bronchoalveolar lavage fluid from RT-PCR-positive patient (ICU-06 in Spike tree=WIV04/2019 virus)successfully isolated nCoV-2019124 BetaCoV/Wuhan/WIV04/2019 in Vero and Huh7 cellscytopathic effects (CPE) seen
🦠culture supernatant (s/n) tested (+) by RT-PCR
🦠rabbit antisera against a bat SARS-related coronavirus (SARSr-CoV) reacted with infected cells
🦠electron microscopy (EM) images of virus particles from infected VeroE6 cells
culture supernatant (s/n) tested (+) by RT-PCR rabbit antisera against a bat SARS-related coronavirus (SARSr-CoV) reacted with infected cellselectron microscopy (EM) images of virus particles from infected VeroE6 cells
culture supernatant (s/n) tested (+) by RT-PCR rabbit antisera against a bat SARS-related coronavirus (SARSr-CoV) reacted with infected cellselectron microscopy (EM) images of virus particles from infected VeroE6 cells
🦠antisera from other infected patients neutralised the isolated virus
Study2: 09MAR2020
https://www.mja.com.au/journal/2020/212/10/isolation-and-rapid-sharing-2019-novel-coronavirus-sars-cov-2-first-patient
🧫nasopharyngeal swab from RT-PCR positive patient
🧫isolated using Vero/hSLAM cell line
🦠CPE seen, virions seen by EM of culture
🦠RT-PCR showed ⬆️viral RNA levels over time in culture
Study2: 09MAR2020 https://www.mja.com.au/journal/2020/212/10/isolation-and-rapid-sharing-2019-novel-coronavirus-sars-cov-2-first-patientnasopharyngeal swab from RT-PCR positive patientisolated using Vero/hSLAM cell lineCPE seen, virions seen by EM of culture RT-PCR showed viral RNA levels over time in culture
Study2: 09MAR2020 https://www.mja.com.au/journal/2020/212/10/isolation-and-rapid-sharing-2019-novel-coronavirus-sars-cov-2-first-patientnasopharyngeal swab from RT-PCR positive patientisolated using Vero/hSLAM cell lineCPE seen, virions seen by EM of culture RT-PCR showed viral RNA levels over time in culture
Study2: 09MAR2020 https://www.mja.com.au/journal/2020/212/10/isolation-and-rapid-sharing-2019-novel-coronavirus-sars-cov-2-first-patientnasopharyngeal swab from RT-PCR positive patientisolated using Vero/hSLAM cell lineCPE seen, virions seen by EM of culture RT-PCR showed viral RNA levels over time in culture
🦠whole genome sequencing of the virus from cell culture supernatants was successful (BetaCoV/Australia/VIC/01/2020)
Study3: 19FEB2020
https://ophrp.org/journal/view.php?doi=10.24171/j.phrp.2020.11.1.02
🧫nasopharyngeal and oropharyngeal samples used
🧫isolated using Vero cells
🦠CPE seen, virions seen by EM of culture
🦠RT-PCR showed ⬆️viral RNA levels over time in culture
🦠whole genome sequencing of the virus from cell culture supernatants was successful BetaCoV/Korea/KCDC03/202
Study4: 09JUN2020
https://www.medrxiv.org/content/10.1101/2020.06.08.20125310v1
🧫swab from upper airways & sputa (n=23)
🧫isolated using Vero clone 118 cells
🦠CPE was seen
🦠rabbit antisera against a SARS-CoV NP reacted with infected cells
Study5: 24APR2020
https://www.nejm.org/doi/full/10.1056/NEJMoa2008457
🧫(n=17)
🧫isolated using Ver-CCL-81 cells
🦠CPE was seen
🦠RT-PCR showed used to confirm viral growth
Study6: 18MAY2020
https://wwwnc.cdc.gov/eid/article/26/8/20-0681_article
🧫faecal samples (n=2)
🧫isolated using Vero E6 cells
🦠CPE was seen, virions seen by EM of culture
🦠patient antisera reacted in an immunofluorescent assay
Infectious SARS-CoV-2 in Feces of Patient with Severe COVID-19

Severe acute respiratory syndrome coronavirus 2 was isolated from feces of a patient in China with coronavirus disease who died. Confirmation of infec...

https://wwwnc.cdc.gov/eid/article/26/8/20-0681_article
🦠whole genome sequencing of the virus from cell culture supernatants was successful (NC045512.2)
Also mentioned in media:
🗞️ https://about.unimelb.edu.au/newsroom/news/2020/january/melbourne-scientists-first-to-grow-and-share-novel-coronavirus
🗞️ https://www.scmp.com/news/hong-kong/health-environment/article/3047956/china-coronavirus-hong-kong-researchers-have
🗞️ http://www.xinhuanet.com/english/2020-01/26/c_138734908.htm
🗞️ https://www.independent.co.uk/news/science/coronavirus-covid-19-virus-isolate-canada-scientists-mutations-strains-a9399226.html
🗞️ https://theconversation.com/i-study-viruses-how-our-team-isolated-the-new-coronavirus-to-fight-the-global-pandemic-133675
This is not an exhaustive or in any way complete list of successful culture isolations.
But it CLEARLY shows that infectious, viable, "live" SARS-CoV-2 virus *has* been isolated by different labs & from different people, all over the world.
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