SiRNA approaches do not seem so bad all of the sudden...
SiRNA approaches do not seem so bad all of the sudden...
SiRNA approaches do not seem so bad all of the sudden...
SiRNA approaches do not seem so bad all of the sudden...
SiRNA approaches do not seem so bad all of the sudden...
And don't get me wrong, I love me some CRISPR (you can ask @LauraTorrente4, @Laura_C_95 or @ritadorta ... ;). But to study stress responses (e.g to DNA damage) especially in p53 WT cells, maybe not the best idea? And I don't even want to think about KRAS, VHL and others...
BTW, when you use siRNAs, do you use a pool and then deconvolute? And if so, how variable would you say your effects are among the different siRNAs (even obtaining a similar knockdown efficiency)?
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