Because I do believe that it's important to know the history of your field and inspired by @carlzimmer @matthewcobb & Michel Morange's books that contain some fantastic material for teaching, a thread with some #classicpaper in #molecularbiology #DNA
1⃣ 1944 Oswald Avery, Colin MacLeod & Maclyn McCarty - DNA, not protein as was commonly believed, is the hereditary material for bacteria, and the cause of bacterial transformation
2⃣ 1947 André Boivin & Roger Vendrely - a near forgotten 2 pages in French that suggest almost explicitly that DNA --> RNA --> protein
3⃣ 1952 & 1953 Alexander Dounce - like Boivin & Vendrely, Dounce is one of the first to propose that DNA might serve as a template for the synthesis of RNA, which in turn serves as a template for the synthesis of proteins
4⃣ 1952 Alfred Hershey & Martha Chase - They confirmed that DNA was the molecule of heredity  a.k.a as the blender experiment
5⃣ 1953 The structure of DNA - Watson & Crick, Franklin & Gosling, Wilkins Stokes Wilson
6⃣ 1956 & 1958 Francis Crick - The Central Dogma: once ‘information’ is passed into protein it cannot get out again
7⃣ NOT the Central Dogma DNA -> RNA -> Protein (for more info see
8⃣ 1958 Francis Crick - The adaptor hypothesis: to explain how information encoded in DNA is used to specify the amino acid sequence of proteins
1⃣0⃣ Vernon Ingram - The first demonstration that the abnormal haemoglobin in sickle cell anaemia patients is caused by an alteration in one amino acid
1⃣1⃣ 1958 Matthew Meselson and Franklin W. Stahl - experimental proof of Semi-Conservative DNA Replication
1⃣2⃣1959 Pardee, Jacob & Monod a.k.a The PaJaMo experiment that supported the hypothesis that a molecule mediated the production of proteins from DNA (cytoplasmic messenger)
1⃣3⃣ 1961 Jacob & Monod - the fundamental basis of gene regulation, one of the most influential paper in the history of modern biology (& I am not saying that because Jacob & Monod were French 😬)
1⃣5⃣ for an historical point of view of the discovery of mRNA, see also this great recount by @matthewcobb
1⃣6⃣ 1961 Marshall W. Nirenberg & J. Heinrich Matthaei - A poly-U RNA was translated into polyphenylalanine in a cell-free system. This experiment provided the initial clue to breaking the genetic code &
1⃣7⃣ 1961 Crick, Barnett, Brenner & Watts-Tobin - The existing knowledge in 1961 & the experimental procedures were certainly not sufficient to allow anyone to deduce the general nature of the genetic code but they nearly solved the riddle
1⃣8⃣ 1965 Margarita Salas - The first experimental results indicating that the direction of reading of the genetic message is from the 5’ to the 3’ end  (see also My scientific life by Margarita Salas in 2016
1⃣9⃣ 1964 K. Marcker & F. Sanger and 1966 B. F. C. Clark & K. A. Marcker - a role for methionine in polypeptide chain initiation.
2⃣0⃣ 1966 Francis Crick - The Wobble hypothesis. A visionary Crick again explains why multiple codons can code for a single amino acid
2⃣2⃣ 1969 Britten & Davidson - Like the Monod & Jacob paper in 1961, a very influential paper on gene regulation. Their theory stated the hypothesis that repetitive non-coding sequences are at the core of genetic regulation
2⃣3⃣ 1968 Karin Ippen-Ihler - Studies using the lac operon identified the
promoter as a cis controlling element for gene transcription
2⃣4⃣ 1969 Bob Roeder & William J. Rutter - the discovery of 3 chromatographically separable forms of eukaryotic RNA polymerase from sea urchin embryos (I, II and III)
2⃣7⃣ 1975 P. Oudet, M. Gross-Bellard, & P. Chambon - the first electron microscopy of reconstituted histone–DNA complexes a.k.a. beads on a string
2⃣9⃣ 1980 Corden - One of the first comparisons of promoter sequences from efficiently transcribed protein-coding genes
OK I think I will stop here, thanks all for your interest in this thread and for all you very positive feedback #classicpaper
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